Applied Biosystems StepOne および StepOnePlus

1 Applied Biosystems StepOne StepOnePlus Real-Time PCR Systems Applied Biosystems StepOne StepOnePlus Real-Time PCR Systems Copyright 2007, 2010 Applied Biosystems . All rights in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this Biosystems DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL Applied Biosystems BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE Research Use Only.

2 Not for use in diagnostic TO PURCHASER: Label License The StepOne and StepOnePlus Real-Time PCR Systems are covered by US patents and corresponding claims in their non-US counterparts. No right is conveyed expressly, by implication, or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5 nuclease methods. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems , 850 Lincoln Centre Drive, Foster City, California 94404, TO PURCHASER:PLEASE REFER TO THE USER'S GUIDE OR PRODUCT INSERT OF THE REAGENTS NAMED HEREIN FOR LIMITED LABEL LICENSE OR DISCLAIMER : Applied Biosystems , AB (Design), MicroAmp, Primer Express, and VIC are registered trademarks, and FAM, JOE, MicroAmp, MultiScribe, NED, ROX, StepOne , StepOnePlus , TAMRA, TET, and VeriFlex are trademarks of Applied Biosystems or its subsidiaries in the and/or certain other countries.

3 AmpErase, AmpliTaq Gold, and TaqMan are registered trademarks of Roche Molecular Systems, is a registered trademark of Molecular Probes, other trademarks are the sole property of their respective owners. 4377716 Rev. C 06/2010 iiiApplied Biosystems StepOne StepOnePlus Real-Time PCR Systems .. vii .. ix .. xii 1 StepOne StepOnePlus .. 1-2 .. 1-4 .. 1-6 .. 1-7 .. 1-8 .. 1-9 2 .. 2-2Ta q M a n .. 2-2 SYBR Green .. 2-4 .. 2-6 DNA .. 2-7 3 : .. 3-3 .. 3-4 .. 3-51 2 RT-PCR .. 3-8 PCR .. 3-10 .. 3-12 .. 3-12.

4 3-15 Inventoried/Made to Order .. 3-16 TaqMan Gene Expression Assays .. 3-16ivApplied Biosystems StepOne StepOnePlus Real-Time PCR Systems Custom TaqMan Gene Expression Assays .. 3-18 .. 3-19 .. 3-20 .. 3-21 Primer Express Software .. 3-21 .. 3-24 .. 3-27 .. 3-30 .. 3-33 .. 3-35 4 : .. 4-3 .. 4-4 .. 4-6 : .. 4-9 .. 4-10 TaqMan SNP Genotyping Assays .. 4-10 TaqMan Drug Metabolism Genotyping Assays .. 4-11 Pre-Developed TaqMan Assay Reagents for Allelic Discrimination .. 4-12 .. 4-13 .. 4-14 .. 4-14 Custom TaqMan SNP Genotyping Assays.

5 4-14 .. 4-16 .. 4-16 5 : .. 5-3 .. 5-4 .. 5-6 : .. 5-9 Inventoried/Made to Order .. 5-10 TaqMan Gene Expression Assays .. 5-10 Custom TaqMan Gene Expression Assays .. 5-12 TaqMan Exogenous Internal Positive Control Reagents .. 5-13 .. 5-15 .. 5-16 .. 5-16vApplied Biosystems StepOne StepOnePlus Real-Time PCR Systems A .. A-2 .. A-5 CT CT .. A-7 B PCR C D .. D-2 Inventoried/Made to Order .. D-2 .. D-2 .. D-5 .. D-5 .. D-5 .. D-7 Inventoried/Made to Order .. D-7 .. D-8 viApplied Biosystems StepOne StepOnePlus Real-Time PCR Systems viiApplied Biosystems StepOne StepOnePlus Real-Time PCR Systems Applied Biosystems StepOne StepOnePlus Real-Time PCR Systems StepOne StepOnePlus Applied Biosystems StepOne StepOnePlus Real-Time PCR Systems Applied Biosystems StepOne Real-Time PCR System Applied Biosystems StepOnePlus Real-Time PCR System 2 PN PNApplied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Getting Started Guide for Genotyping ExperimentsStepOne StepOnePlus 2 Applied Biosystems StepOne Real-Time PCR Software StepOne

6 StepOne StepOnePlus 43767864377731 Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Getting Started Guide for Presence/Absence Experiments43767874377722 Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Getting Started Guide for Relative Standard Curve and Comparative CT Experiments43767854377740 Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Getting Started Guide for Standard Curve Experiments43767844377734 Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Installation, networking , and Maintenance GuideStepOne StepOnePlus StepOne StepOnePlus 43767824377796 Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Installation Quick Reference Card43767834377790 Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Reagent GuideStepOne StepOnePlus TaqMan SYBR Green StepOne StepOnePlus 43797044377716viiiApplied Biosystems StepOne StepOnePlus Real-Time PCR Systems Microsoft Windows XP DNA RNA PCR

7 StepOne StepOnePlus 0 Enter File Open Applied Biosystems 2 Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Site Preparation GuideStepOne StepOnePlus StepOne StepOnePlus 43767684378357 Applied Biosystems StepOne Real-Time PCR Software HelpStepOne StepOne StepOnePlus StepOne StepOnePlus StepOne StepOnePlus RNase P StepOne StepOnePlus StepOne StepOnePlus StepOne StepOnePlus PN PNixApplied Biosystems StepOne StepOnePlus Real-Time PCR Systems Calibrate ID StepOne StepOnePlus StepOne StepOnePlus 2007 7 PNApplied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Installation Performance Verification

8 Protocol4376791 Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Installation Qualification-Operation Qualification Protocol4376790 Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Planned Maintenance Protocol4376788 PNAllelic Discrimination Pre-Developed TaqMan Assay Reagents Quick Reference Card4312212 Custom TaqMan Genomic Assays Protocol4367671 Custom TaqMan SNP Genotyping Assays Protocol4334431 Ordering TaqMan SNP Genotyping Assays Quick Reference Card4374204 Performing a Custom TaqMan SNP Genotyping Assay for 96-Well Plates Quick Reference Card4371394 Performing a TaqMan Drug Metabolism Genotyping Assay for 96-Well Plates Quick Reference Card4367636 Pre-Developed TaqMan Assay Reagents Allelic Discrimination Protocol4312214Ta q M a n Drug Metabolism Genotyping Assays Protocol4362038Ta q M a n SNP Genotyping Assays Protocol4332856xApplied Biosystems StepOne StepOnePlus Real-Time PCR Systems CT PNDNA Isolation from Fresh and Frozen Blood, Tissue Culture Cells, and Buccal Swabs Protocol 4343586 NucPrep Chemistry.

9 Isolation of Genomic DNA from Animal and Plant Tissue Protocol4333959 PrepMan Ultra Sample Preparation Reagent Protocol4318925 PNAmplification Efficiency of TaqMan Gene Expression Assays Application Note127AP05 Applied Biosystems High-Capacity cDNA Reverse Transcription Kits Protocol4375575 Custom TaqMan Gene Expression Assays Protocol4334429 Primer Express Software Version Getting Started Guide4362460Ta q M a n Gene Expression Assays Protocol4333458 User Bulletin #2: Relative Quantitation of Gene Expression4303859 PNAmplification Efficiency of TaqMan Gene Expression Assays Application Note127AP05 Custom TaqMan Gene Expression Assays Protocol4334429 Primer Express Software Version Getting Started Guide4362460Ta q M a n Gene Expression Assays Protocol4333458 User Bulletin #2.

10 Relative Quantitation of Gene Expression4303859xiApplied Biosystems StepOne StepOnePlus Real-Time PCR Systems xii StepOne F1 Help StepOne Software Help StepOne PNApplied Biosystems High-Capacity cDNA Reverse Transcription Kits Protocol4375575 Custom TaqMan Gene Expression Assays Protocol4334429 Custom TaqMan Genomic Assays Protocol: Submission Guidelines4367671 Custom TaqMan SNP Genotyping Assays Protocol4334431 Power SYBR Green PCR Master Mix and RT-PCR Protocol4367218 Pre-Developed TaqMan Assay Reagents Allelic Discrimination Protocol4312214 Primer Express Software Version Getting Started Guide4362460 SYBR Green PCR and RT-PCR Reagents Protocol4304965 SYBR Green PCR Master Mix and RT-PCR Reagents Protocol4310251Ta q M a n Drug Metabolism Genotyping Assays Protocol4362038Ta q M a n Exogenous Internal Positive Control Reagents Protocol4308335Ta q M a n Fast Universal PCR Master Mix (2 )