Transcription of ˘ˇˆ˙ Clostridium - jscm.org
1 E *Y/+. 2010 9.. Clostridium . !"1)# $%. 2)# 2). 1). & ' ( ) * +, 2). - ./ ,. 01234567 # 12 . 8 9 21 6 : 19 ; < 9 21 11 : 2 ; =. >? @ A B CD EF G H!IJK Clos- tridium K < L /M. N K IO PQ "#BR$ NI%S . TU H!&'(< V)*'W+ X%YZ 16S ribosomal RNA [,\-. ]^ . T Clostridium hathewayi, Clostridium clostridioforme, Clostridium bolteae, Clostridium citro- niae, Clostridium aldenense, Clostridium symbiosum 6 _`< a/ b (API 20. A, Rapid ID32A) I0# T/M. N%YZcd1e Yfg YC R$ TU Indole W/ _` C. citroniae C. aldenense hif< j 2 _ R$ N. `< rhamnose, b-glucuronidase, b-galactocidase @2 TU Indole 3W/ 4 _ Ck C. symbiosum l@ a-galactocidase himTU C. hathewayi `< cellobiose 4 < a-arabinosidase %.
2 YZ b-N-acetylglucosaminidase 4 YfR$n5himTU C. clostridioforme C. bol- teae R$ K `< N@6o %fR$` p7himTU qT< C. symbiosum `. cd1eI# Br Js8H!tAR$n5himTU. Key words: Clostridium clostridioforme, Clostridium bolteae, Clostridium hathewayi, Clos- tridium citroniae, Clostridium aldenense B Clostridium symbiosum5 8) @i U t i `9u . v wx y A< C. clostridioforme T _`< LCM. BH! z{ Clostri- B NI h< 16S ribosomal RNA (16S. dium clostridioforme `< | : h ` * } ~ ; B rRNA) [,\ -. X Y \/+.& N . Clostridium < K. %f1)< = > N O tA < C. clos- @ A j 2) * ?tA @ @ Clostri- tridioforme, Clostridium bolteae9), Clostridium hathe- dium < % 2 AB C j 3) @D wayi 10 14) 3 _ 6 T@1)< <.]
3 U E % `< j qh' B A 6 W / @ _ Clostridium @< v v 4)hJ< citroniae15) %YZ Clostridium aldenense15) P. Clostridium ` @ E TU j A 5 _` C. clostridioforme group tA @ < = > J TU 1, 16)U T@m < . Clostridium `< C. clostridio- C Clostridium ` C. forme H!tA`C. clostridioforme R$@p7 symbiosum I 6 _@Q j B U. C. clostridioforme `j YC 6 F GH ( 629 2261) & ' ' IJ = ; Rh Bf2, 4, 17)< j A. 481. I @i . DA U qT< & . & ' ( ) * +, !" S T Yf"#B DIU . CzRt TEL: 0772 46 3371 8K 3150= A< V&B * + hJn5B _$i `i FAX: 0772 46 3371 WX _ qh N@ h . E *Y/+. Vol. 20 No. 1 2010. 9. 10 . 10 Vol. 20 No. 1 2010. Clostridium clostridioforme group 2 11. saminidase (b-NAG) 8 }1W= 3 >$~.
4 0 m 20A W#'h5/ 72 h5~0. !"#$ %%& m *+, W ? @ C. clos- ' ( ) *+, api 20a - tridioforme 1 ?t .. Rapid ID32A /. 0 1( %2 0 1 *+, C. clostridioforme ~ . xA 2 0 .. 1. & '. 2004 11 3% 2006 9 3 23 435 6 1. (Fig. 1). 278 9 : ;< =: g #' !"# -i @ W . >? % @ AB I+, K WB ! C DEFGH, IJK L MN $C @ D % ! W.. O - . 16 S ribosomal RNA P Q (1,486 R % j(/. UW 26 U (43 ) . 1,489 bp) ! $ 2 S Q T C. E W C. hathewayi (63 ) C. citroniae (67 ). hathewayi 16 U C. clostridioforme 10 U C. bolteae j(@ A1 % 1 C. aldenense (20 ) C. 11 U C. citroniae 6 U C. aldenense 5 U C. sym- symbiosum (17 ) WF% (Table 2) 60 U . biosum 12 U V 60 U/ - / AC #$ nopqr 2. 24 h5 48 h5 72 h5#'0 u,/G 0.)))
5 W XYGZG[\X]^_;<` u,1-w U~ U1H . "#$ aAC #$ %&bF,[cdJ+*[e[f 0 72 h5#' W 48 UWI 3R5 mm u, g #'h a3 %5#'f !"# -i " / 12 UW 2 mm r D % u, #$ @ - .j( )*/kl0 m +2 /@ 0 E1( ~ u,/. U/ AC #$ nopqr s #'0 24 h5 @ 0 UW C. symbiosum @ . 48 h5 72 h5#'t u,$v a-w @ W 24 h5 #' WJ . f % @ K 1 L 0 u, -w u 3. !"#$ % , W-w k~ Bacteroides ? 0 . y / 0z /{|}1~0 *+, API 1 72 h5 #' W 0 J 1M N~. 20A a20A %&\ Kf ~z23 /y4 (Fig. 1) #OW ' P# W . {|}1~0 Rapid ID32A a32A f / # MQD C. symbiosum 5 . 5- . $v/6 789 Wu,@ ! /. % . 0 : W ' 1 GI J 2. ( )*+,$ Clostridium $ . ; ~~ / <0 32A W a- - .$/0(12+$34. galactosidase (a-GAL), b-galactosidase (b-GAL), b- 2 0 R U *+, 32A.))]]]
6 20A. galactosidase-6-phosphate (b-GP), a-glucosidase (a- 6 5/ Table 1 0 . GLU), b-glucosidase (b-GLU), a-arabinosidase (a- C. hathewayi 16 U 20 A - i x A W. ARA), b-glucuronidase (b-GUR), b-N-acetylgluco- 32A W ~ xA. Fig. 1. Cellular morphologies and colonies of C. clostridioforme group and C. symbiosum a) Spore stain of C. hathewayi b) Gram stain of C. clostridioforme c) Gram stain of C. bolteae d) Gram stain of C. citroniae e) Gram stain of C. aldenense f) Gram stain of C. symbiosum g) C. clostridioforme group and C. symbiosum on Anaero-Columbia agar with rabbit blood after 72. h of incubation. Colonies of C. clostridioforme group (right) are circular with slight irregular edges and attained a diameter of 3 5 mm.
7 In comparison, C. symbiosum (left) forms small colony. %& >= Vol. 20 No. 1 2010. 11. Table 1. Identi cation by use of api 20a and Rapid ID 32A. 12. api 20a Rapid ID32A. Species (No. of isolates) Species and ID obtained No. of No. of Pro le No. Pro le No. Species and ID obtained by Rapid ID32A. by api 20a isolates isolates C. hathewayi (16) 46746301 C. clostridioforme 1 4571040000 C. clostridioforme 1. 46756330 C. clostridioforme 2 4575044000 C. clostridioforme 1. 46756331 C. clostridioforme 2 4577040000 C. clostridioforme 5. 46756730 C. clostridioforme 4 4577044000 C. clostridioforme 6. 46756731 C. clostridioforme 7 4577044020 C.
8 Clostridioforme 3. 12 . C. clostridioforme (10) 00100020 P. propionicus/avidum 1 4150000000 C. baratii , C. clostridioforme 3. 00604000 Fusobacterium mortiferum 1 4550000000 C. clostridioforme , C. beijerinckii/butyricum 5. 40044231 C. clostridioforme 1 4550040000 C. clostridioforme 2. Vol. 20. 42144231 C. clostridioforme, B. distasonis 1. 42644030 1. 44044230 Fusobacterium mortiferum 1. 44704020 Fusobacterium mortiferum 1. No. 1. 46754010 B. distasonis 1. 46754031 C. clostridioforme 1. 46754230 C. clostridioforme 1. 2010. C. bolteae (11) 00000000 4 4500000000 C. beijerinckii/butyricum 3. 00000001 3 4540000000 C. beijerinckii/butyricum , C.
9 Fallax , C. clostridioforme 7. 04514331 C. clostridioforme 1 4750000000 C. tertium , C. beijerinckii/butyricum , C. baratii 1.. 20010020 B. ureolyticus 1. 44104331 C. clostridioforme 1. 44754031 C. clostridioforme 1. C. citroniae (6) 10000010 F. necrophorum/nucleatum 1 0040200000 F. necrophorum 1. 10100031 C. botulinum/sporogenes 1 0040600000 F. necrophorum 4. 54104030 P. intermedia/disiens 1 4040200000 F. necrophorum 1. 54144031 C. clostridioforme 1. 54554031 C. clostridioforme 1. 54556031 C. clostridioforme 1. C. aldenense (5) 10000000 F. necrophorum/nucleatum 1 4100200000 C. clostridioforme 1. 10454200 B. stercoris/eggerthii 1 4120200000 C.
10 Clostridioforme 2. 14510201 C. clostridioforme 1 4120600000 C. clostridioforme 2. 50554220 B. stercoris/eggerthii 2. C. symbiosum (12) 00000000 6 0000000000 1. 00100000 P. intermedia/disiens 1 0100000000 C. septicum , C. fallax , C. botulinum 11. 40010001 C. cadaveris 1. 41000000 E. limosum 2. 41010001 C. cadaveris 1. 41014000 P. propionicus/avidum 1. Table 2. Results of biochemical reaction in commercial identi cation kits for strains identi ed by 16S rRNA sequencing Rapid api 20a . Species Strain ID 32A. pro le IND. IND. ESC. CEL. SAC. SAL. SRO. PAL. L AC. TRE. GLU. RAF. (No. of isolates) No. pro le RAF. LGA. XYL. ML Z. RHA. ARA.
