QuantStudio 6 and 7 Flex Real-Time PCR Systems

1 USER GUIDEQ uantStudio 6 and 7 flex Real-Time PCR SystemsMAINTENANCE AND ADMINISTRATIONP ublication Number4489821 RevisionANovember 2013 For Research Use Only. Not for use in diagnostic information in this guide is subject to change without TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED BY LAW, IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE USE LABEL LICENSE No.

2 477: Real-Time PCR SystemNotice to Purchaser: This product is licensed for use under certain patent claims owned by the University of Utah Research Foundation and licensed to BioFire Diagnostics, Inc. No right is conveyed, expressly, by implication or by estoppel under any other patent claim. TRADEMARKSThe trademarks mentioned herein are the property of Life Technologies Corporation and/or its affiliate(s) or their respective Explorer, Excel, Microsoft, Windows Vista and Windows are registered trademarks of Microsoft Corporation. Cy is a registered trademark of GE Healthcare. Safari, Apple and Macintosh are registered trademarks of Apple, Inc. Mozilla and Firefox are registered trademarks of Mozilla Foundation.

3 Adobe and Acrobat are registered trademarks of Adobe Systems , Inc. Twister is a registered trademark of Calliper Life Sciences, Inc. TaqMan is a registered trademark of Roche Molecular Systems , Inc., used under permission and license. 2013 Life Technologies Corporation. All rights 6 and 7 flex Real-Time PCR Systems Maintenance and Administration GuideAbout This Guide .. 11 Purpose .. 11 Audience .. 11 Assumptions .. 11 User attention words .. 12 Symbols on this instrument .. 12 Contents of this manual .. 13 CHAPTER 1 Getting Started .. 15 Differences between the QuantStudio 6 and 7 flex Real-Time PCR Systems .. 16 About the QuantStudio 6 and 7 flex Real-Time PCR Systems .. 16 Data collection.

4 16 Instrument filters and supported dyes .. 17 Instrument hardware .. 18 Front .. 18 Rear .. 19 Barcode scanner .. 20 Twister Robot components .. 21 Electrical protective devices .. 23 Instrument software .. 23 Computer requirements .. 24 Software installation .. 24 Third-party software .. 24 Instrument consumables .. 25 Interchangeable sample blocks, heated covers, and plate retainers .. 26 CHAPTER 2 Calibrate the Instrument .. 27 Calibration overview .. 28 Calibration and maintenance schedule .. 28 Planned maintenance .. 28 About maintenance reminders .. 29 Calibration workflow .. 29 View the calibration status of an instrument .. 29 Enable or change the calibration reminders .. 31 Collect the required information.

5 31 Define the mail server settings .. 31 Modify the notification settings for a monitored instrument .. 334 QuantStudio 6 and 7 flex Real-Time PCR Systems Maintenance and Administration GuideContentsGuidelines for preparing calibration plates and array cards .. 35 Preparing array cards for instrument calibration .. 36 Required materials .. 36 Filling the calibration array cards .. 36 ROI calibration .. 40 When to perform the calibration .. 40 About the calibration data .. 40 Preparing the calibration plate or array card .. 41 Prepare the ROI calibration plate .. 41 Perform the ROI calibration .. 42 Background calibration .. 44 When to perform the calibration .. 44 About the calibration data .. 44 Preparing the calibration plate or array card.

6 44 Prepare the background calibration plate .. 45 Perform the background calibration .. 46 Uniformity calibration .. 48 When to perform the calibration .. 48 Preparing the calibration plate or array card .. 48 Prepare the ROI calibration plate .. 48 Perform the uniformity calibration .. 49 Dye calibration .. 52 When to perform the calibration .. 52 About the dye calibration data .. 52 Preparing the calibration plate or array card .. 53 Prepare the dye calibration plates .. 53 Perform the dye calibration .. 54 Normalization calibration .. 57 When to perform the calibration .. 57 About the calibration data .. 57 Preparing the calibration plate or array card .. 57 Prepare the normalization calibration plates .. 57 Perform the normalization calibration.

7 58 Verify the instrument performance .. 61 When to perform the test .. 61 About the RNase P kits .. 61 Analytical performance .. 62 Installation specification .. 63 Prepare the RNase P plate .. 63 Preparing an array card for instrument verification .. 64 Run the RNase P plate or array card .. 66 Troubleshooting .. 69 How to identify contamination .. 78 Remove a plate stuck within the instrument .. 785 QuantStudio 6 and 7 flex Real-Time PCR Systems Maintenance and Administration GuideContents APPENDIX A Manual Instrument Operation .. 79 Overview of instrument touchscreen functions .. 80 Operate the instrument from the touchscreen .. 81 Create an experiment from a template .. 81 Run an experiment .. 82 Transfer experiments, templates, and results data.

8 83 Maintain the instrument from the touchscreen .. 85 Back up and restore the instrument settings .. 85 Perform an instrument self test .. 86 Update the instrument firmware .. 87 Administrate the instrument from the touchscreen .. 88 Define the date and time .. 88 Define the instrument settings .. 88 Define the maintenance reminders .. 90 Define the network settings .. 90 Define the system shortcuts .. 91 Review the instrument statistics .. 91 Review the instrument version information .. 91 Enable/disable instrument security .. 92 View the instrument log .. 92 APPENDIX B Maintenance .. 95 Decontaminate the sample block .. 96 Materials required .. 96 How to handle the sample block .. 96 Clean the sample block .. 97 Replace the halogen lamp.

9 99 Materials required .. 99 Halogen lamp warnings .. 99 View the lamp status .. 99 Replace the lamp .. 100 Replace the instrument fuses .. 102 Materials required .. 102 Replace the fuses .. 102 Install the QuantStudio 6 and 7 flex Real-Time PCR system Software .. 103 Computer requirements .. 103 Install the software .. 103 Manage QuantStudio 6 and 7 flex Real-Time PCR system Software licenses .. 104 QuantStudio 6 and 7 flex Real-Time PCR system Software license keys and files .. 104 Activate a license .. 104 Install a license file .. 104 Update the QuantStudio 6 and 7 flex Real-Time PCR system Software .. 105 Update the software .. 1056 QuantStudio 6 and 7 flex Real-Time PCR Systems Maintenance and Administration GuideContentsUpdate the Windows operating system .

10 106 Change the sample block .. 106 When to change the sample block .. 106 Materials required .. 106 How to handle the sample block .. 106 Change the sample block .. 107 Change the heated cover .. 108 When to change the heated cover .. 108 Materials required .. 108 How to handle the heated cover .. 108 Change the heated cover .. 109 Change the plate adapter .. 111 When to change the plate adapter .. 111 Materials required .. 111 Change the plate adapter .. 111 APPENDIX C Networking .. 113 Networking overview .. 114 Controlling and monitoring networked QuantStudio 6 and 7 flex Systems .. 114 About the Ethernet port .. 114 Example network layouts .. 115 Networking guidelines and best practices .. 116 Network setup workflow.