user manual Malvern

1 Printed in England MRK0499-02 Malvern Instruments LimitedEnigma Business ParkGrovewood Road, Malvern Worcs, WR14 1XZ, : +44 (0) 1684 892456 Fax: +44 (0) 1684 manualuser manualZetasizerZetasizernano seriesZetasizerMalvernnano series Zetasizer NanoUser manual MAN0485 Issue April 2013 EnglishCopyright 2007, 2009, 2012, 2013 Malvern Instruments Instruments pursues a policy of continual improvement due to technical development. We therefore reserve the right to deviate from information, descriptions, and specifications in this publication without notice. Malvern Instruments shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance or use of this material. No reproduction or transmission of any part of this publication is allowed without the express written permission of Malvern Instruments office: Malvern Instruments Business Park,Grovewood Road, Malvern ,Worcestershire WR14 1 XZUnited + [44] (0)1684-892456 Fax + [44] (0)1684-892789 Zetasizer, Malvern and the 'hills' logo are registered trademarks in the UK and/or other countries, and are owned by Malvern Instruments and M3-PALS are trademarks of Malvern is granted Euro Pat No: 1 154 266 DE is a registered trademark of Microsoft Corporation.

2 Tygon is a registered trademark of Saint-Gobain Corporation. Hellmanex is a registered trademark of Hellma GmbH & Co. NanoPage iIntroduction to this manualIntroduction.. 1-1 About this manual .. 1-1 Access to the instrument.. 1-2 Assumed information .. 1-3 Where to get help.. 1-3 Introducing the Zetasizer NanoIntroduction.. 2-1 About the Zetasizer Nano .. 2-1 Primary measurement techniques .. 2-6 Hardware components .. 2-11 Software overview .. 2-21 Making measurementsIntroduction.. 3-1 Quick guide to making a measurement.. 3-2 Powering up the system .. 3-2 Sample preparation .. 3-3 Selecting and preparing the measurement cell .. 3-3 Making an SOP measurement.. 3-4 Making a manual measurement.. 3-6 The measurement display.. 3-6 Records and Reports - Viewing the ResultsIntroduction.. 4-1 Displaying the results .. 4-1 Table of ContentsTable of ContentsZetasizer NanoPage iiMAN 0485 Sample PreparationIntroduction.. 5-1 Preparing the sample - Size.. 5-1 Preparing the sample - Molecular weight.

3 5-5 Preparing the sample - Zeta potential.. 5-6 Count rate meter .. 5-9 SecurityIntroduction.. 6-1 Initial start-up - set up the administrator.. 6-2 Enabling security .. 6-3 User groups.. 6-3 Users.. 6-5 Measurement file window - WorkspacesIntroduction.. 7-1 Measurement file window .. 7-2 Using SOPsIntroduction.. 8-1 The SOP editor .. 8-3 Creating an SOP.. 8-9 Measurement type selection .. 8-10 Size SOPs .. 8-11 Molecular weight SOPs.. 8-29 Zeta potential SOPs .. 8-38 Flow-mode SOPs.. 8-47 Trend SOPs .. 8-48 Titration SOPs .. 8-50 Extracting an SOP .. 8-50 Modifying an SOP .. 8-51 Distributing an SOP .. 8-51 Zetasizer NanoTable of ContentsZetasizer NanoPage iiiAdvanced featuresIntroduction.. 9-1 Solvent builder.. 9-2 SOP player .. 9-5 Result averaging.. 9-12 Editing the result .. 9-13 Exporting results .. 9-15 Options window.. 9-20 Expert advice.. 9-26 Report designerIntroduction.. 10-1 Overview.. 10-2 Opening an existing report .. 10-4 Creating a new report.. 10-4 Adding elements to the report.

4 10-4 Setting up the report elements.. 10-6 Selecting elements.. 10-16 Aligning and sizing elements.. 10-17 Saving a report.. 10-17A finished report.. 10-18 Viewing the new report .. 10-19 Other information shown on a report .. 10-19 Protecting a report .. 10-19 Size theoryIntroduction.. 11-1 What is Dynamic light scattering?.. 11-1 Operation of the Zetasizer Nano - Size measurements.. 11-6 Molecular weight theoryIntroduction.. 12-1 What is Static light scattering?.. 12-1 The Debye plot.. 12-4 Table of Contents Zetasizer NanoPage ivMAN 0485 Zeta potential theoryIntroduction.. 13-1 What is Zeta potential?.. 13-1 Laser doppler velocimetry.. 13-5 The M3-PALS technique.. 13-7 Operation of the Zetasizer Nano - Zeta potential measurements.. 13-11 Part 1 - Instrument guideZetasizer NanoPage 1-1 1 Introduction to this manualIntroductionThis manual covers the operation of the Zetasizer Nano particle characterisation system. The instrument measures the size of particles contained within a sample, presenting data according to the user s user manual is a companion to the Zetasizer Nano Basic Guide, which gives Health and Safety, maintenance, troubleshooting and other vital information which all users must this manualThe manual contains the general information required by an operator; as well as supervisor information that concentrates on administration and extension of the system s functionality.

5 Topics covered include: An overview of the Zetasizer Nano hardware and software. A guide to making measurements and how to read the results. Sample preparation guidance. Reporting - creating and editing reports. System security. Advanced an Theory section which describes the basic measurement theories involved with the 1 Introduction to this manualPage 1-2 MAN 03171 Warning!The instrument and the samples to be measured may be hazardous if mis-used. Users must read the Health and Safety information in the Basic Guide before operating the to the instrumentThis manual refers to the various people who will have access to the instrument, as Instruments personnelMalvern Instruments personnel (service engineers, representatives, etc.) have full access to the instrument and are the only people authorised to perform all service procedures that may require the removal of the instrument covers. Warning!Unless advised within the content of this manual , removal of the instru-ment covers by unauthorised personnel, even a supervisor, will invalidate the warranty of the instrument.

6 SupervisorThe supervisor is the person responsible for the management and safety of the instrument and its operation. The supervisor also trains the operators and can per-form user maintenance routines. OperatorAn operator is a person trained in the use of the system. The operator can perform some user maintenance routines !Failure to follow these guidelines could result in exposure to hazardous voltages and laser to this manual Chapter 1 Zetasizer NanoPage 1-3 Assumed informationNaming conventionWithin this manual : The Zetasizer Nano is referred to as the optical unit or the instrument . The accessories or sample dispersion units are referred to as MPT-2 Titrator , the vacuum degasser , or as the accessory or the dispersion unit . The combination of the optical unit, one or more accessories or dispersion units and the computer is referred to as the system .Menu commandsSoftware menu commands are referred to in the form main menu-menu item. As an example, the command Configure-New SOP refers to selecting the New SOP item in the Configure menu.

7 Menu commands are shown in bold to get helpThis section describes the available sources of information on the system. The pri-mary sources of information on the system are instrument and accessory manuals and the software s help system. The manuals give an overview of the system as a whole, while the online help system gives more detailed information on the Zeta-sizer software. Some features are described in both the manuals and Help The following manuals are available for the Zetasizer Nano:Basic Guide A concise yet essential guide that provides an introduction to the system and its usage covering Site requirements, Health and Safety and an instrument and accessory overview. Finally a brief guide to making a measurement and view-ing the results is GuideThe primary source of information about the Zetasizer Nano hardware and its use. See About this manual earlier in the chapter. Accessory and dispersion unit guidesSeparate guides are supplied with the various Nano accessories and Titrator disper-sion units.

8 These provide essential information on the connection, operation and maintenance of the dispersion 1 Introduction to this manualPage 1-4 MAN 0317 Help SystemEach key software window has a Help button giving information specific to it. Additional Info Tips are also available whenever is shown - simply move the mouse pointer over this icon to see a relevant supportMalvern Instruments offers several methods of technical support, which are described in this supportNoteThis is the primary support method only use the other methods if this is not available at your contacting your local Malvern Instruments representative - details of all local telephone numbers are available at Always quote the following information: Model and serial number of the instrument. The serial number is shown on the Zetasizer software status bar - rollover the Nano icon to display. The software version. To find this select Help-About in the software - alternatively, move the mouse over the instrument status bar icon.

9 The Appli-cation Version is the main firmware version number to supportContact the International Malvern Helpdesk if the local Malvern Instruments representative is not available: Telephone: +44 (0)1684 891800 or email: help line is primarily English supportMalvern Instruments offers a remote support service, delivered by an internet con-nection. Benefits include fast and efficient fault diagnosis, as well as reducing downtime and costs. Zetasizer NanoPage 2-1 2 Introducing theZetasizer NanoIntroductionThis chapter provides information about the Zetasizer Nano hardware and soft-ware, and identifies all major features of the system. The following topics are covered: A description of the purpose of the instrument, its accessories and simple explanations of the primary measurement techniques Detailed reference on the physical parts of the optical unit. An overview of the primary software features. Information about the parts of the cell, including how to insert and remove it.

10 The following topics are covered: NoteHardware maintenance - for details on how to maintain the hardware, please refer to the Basic Guide and the Accessory will be made throughout the manual to the MPT-2 Titrator and other accessories. Refer to the MPT-2 Titrator and Degasser, and the Zetasizer Nano accessories user manuals where the Zetasizer NanoThe Zetasizer Nano range of instruments provides the ability to measure three characteristics of particles or molecules in a liquid three fundamental parameters are particle size, zeta potential and molecular weight. Unique technology within the Zetasizer system allows these parameters to be measured over a wide range of concentrations. Depending upon Chapter 2 Introducing the Zetasizer NanoPage 2-2 MAN 03172 the options and accessories used, the Zetasizer system also has the ability to per-form: Microrheology measurements Autotitration measurements if an MPT-2 accessory is connected, Trend measurements, including the determination of the Aggregation Zetasizer range features pre-aligned optics and programmable measurement position for the measurement of size and zeta potential over a wide concentration range and precise temperature control necessary for reproducible, repeatable and accurate measurements.