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BIOCHEMICAL CHARACTERIZATION OF THERMOPHILIC …
, VOL. 2(3) 2011: 616 - 620 ISSN 2229 6441 616 BIOCHEMICAL CHARACTERIZATION OF THERMOPHILIC amylase ENZYME ISOLATED FROM BACILLUS STRAINS Elhadi A. I. Elkhalil and Fatima Y. Gaffar Department of Botany & Agric. Biotechnology, Faculty of Agriculture, University of Khartoum, 13314 Shambat, SUDAN ABSTRACT This study was conducted to screen for thermophillic Bacillus strains with amylase activity and to examine the amylase heat tolerance potentiality. It was, also this study was aimed to determine the optimal culture conditions for growth and amylase production. Two strains were isolated from soil: Bacillus sterothermophilus and Bacillus acidocaldarius. Two growth media were prepared to determine the optimal condition for amylase production, one was complex media containing starch , yeast extract , (NH4)2 HPO4 , KCl and MgSO47H2O , and the other was semi-synthetic medium containing peptone , (NH4)2 HPO4 , NH4H2PO4 and KCl The two isolates grew better on complex medium than on semi synthetic medium and amylase production on complex medium was highly expressed ranging between U/ml.
Biochemical characterization of thermophilic amylase enzyme isolated from Bacillus strains 617 BIOCHEMICAL CHARACTERIZATION OF AMYLASE Determination of pH optima
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