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Western blot procedure - Abcam
2. Sample preparation 2.1. Remove a small volume (50 µl) of lysate to perform a protein assay. Determine the protein concentration for each cell lysate. 2.2. To the remaining volume of cell lysate, add an equal volume of 2 X Laemmli Sample Buffer. We recommend to reduce and denature the sample using the following method unless the online
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