TRANSFECTION PROTOCOL Lipofectamine 3000 …
On the day of transfection, which should be 1 day following cell plating, perform the following steps, which have been optimized for a single well of a 24-well plate using Invitrogen ™ Lipofectamine 3000 Transfection Reagent: Step Tube Complexation components Amount per well (24-well) 1 Tube 1 Opti-MEM™ I medium 25 µL
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