Western blot procedure - Abcam
antibody datasheet directs that non-reducing and non-denaturing conditions should be used: 2.3 To reduce and denature: Boil each cell lysate in sample buffer at 100oC for 5 min and aliquot. Store lysates at -20oC. Note: aliquot cell lysates (50- 100 µl) to avoid repeat freeze/thaw cycles.
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