Design and optimization of SYBR Green assays
of a single PCR product. Unfavorable result: multiple peaks indicative of more than one PCR product. SYBR Green dye will bind to both products and produce a signal. Reverse primer Shortcut See page 3 for information on how to eliminate all bioinformatics steps. Example sequence for RAS
Download Design and optimization of SYBR Green assays
Information
Domain:
Source:
Link to this page:
Documents from same domain
Determination of Trace Elements in Steels and …
assets.thermofisher.comdetermination of minor and trace elements simultaneously, without the need for additional dilution or pre-concentration techniques, resulting in time and cost efficient sample preparation and analysis.
Product information: RevertAid First Strand cDNA …
assets.thermofisher.comPRODUCT INFORMATION Thermo Scientific RevertAid First Strand cDNA Synthesis Kit #K1622 100 rxns Lot __ Expiry Date __ Store at -20°C d www.thermoscientific.com/onebio
Ion OneTouch 2 System - Thermo Fisher Scientific
assets.thermofisher.comIon OneTouch™ 2 System USER GUIDE ... • Ion OneTouch™ Sippers • Ion OneTouch™ 2 Amplification Plates See product web pages ... In the instrument touch screen, touch Options, then touch Info. Firmware updates can only be installed by Administrator-level users on the Torrent
Ion Sphere Assay on the Qubit 3.0 Fluorometer
assets.thermofisher.comIon Sphere™ Assay on the Qubit™ 3.0 Fluorometer User Guide Ion Sphere™ Assay overview ... Microcentrifuge MLS Unenriched ISPs Provided by user ... Touch Ion Sphere on the Qubit™ 3.0 Fluorometer home screen open the Ion Sphere™ Assay. Touch AF 488. Download the
Quantifiler HP and Trio DNA Quantification Kits …
assets.thermofisher.comThe Quantifiler™ HP and Trio DNA Quantification Kits User Guide provides information about Thermo Fisher Scientific instruments, chemistries, and software associated with the Quantifiler ™ HP and Trio DNA Quantification Kits.
For Research Use Only. CAUTION: Not for human or animal ...
assets.thermofisher.comResearch Use Only In vitro diagnostic medical device Use by Temperature limitation Manufacturer European Community authorised representative [-] Without, does not contain [+] With, contains Protect from light Consult accompanying documents Directs the user to consult instructions for use (IFU), accompanying the product. ...
Research Use Only - assets.thermofisher.com
assets.thermofisher.comBy use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use
Connected chromatography solutions
assets.thermofisher.comSelection of the correct GC columns and accessories is critical to ensure optimum system performance. The selection guide below is designed to simplify this process Vials and Closures
Ubuntu Self-Upgrade kit for Torrent Servers
assets.thermofisher.comUbuntu Self-Upgrade kit for Torrent Servers ... Upgrading your server to Ubuntu 14.04 is a two-part process: • Download the software and create a bootable USB drive with Ubuntu 14.04 and Torrent Suite Software. ... The information in this guide is subject to change without notice.
Thermo Scienti c EMCPro Plus EMC Test System
assets.thermofisher.comThe Thermo Scienti c EMCPro Plus EMC Test System features resident capabilities for EMC CE Mark compliance testing to IEC/EN standards, and fully addresses new requirements
Related documents
Introduction to Quantitative PCR - Agilent
www.agilent.comJun 24, 2016 · (endpoint semi-quantitative PCR) or while the amplification is still progressing (real-time QPCR). In endpoint semi-quantitative PCR, fluorescence data are collected after the amplification reaction has been completed, usually after 30–40 cycles, and this final fluorescence is used to back-calculate the amount of template present prior to PCR.
QPCR Optimization & Troubleshooting Guide
assets.thermofisher.comValidate performance of PCR primers using SYBR Green. Redesign probe or optimize probe binding step if primers are performing well. Probe exposed to light and been bleached Once reaction set up is complete return your probe to the product packaging as soon as possible to mimimize exposure to light. 04 Troubleshooting Guide
RT-qPCR guidelines - Real-time PCR Research and ...
pcrlab.vetmed.ucdavis.eduFor SYBR Green I, Cq of the NTC E Calibration curves with slope and y intercept E PCR efficiency calculated from slope E CIs for PCR efficiency or SE D r2 of calibration curve E Linear dynamic range E Cq variation at LOD E CIs throughout range D Evidence for LOD E If multiplex, efficiency and LOD of each assay E
QUANTITATIVE RT-PCR PROTOCOL (SYBR Green I)
schnablelab.plantgenomics.iastate.eduApr 01, 2007 · Quantitative RT-PCR Protocol (SYBR Green I) 5 (3) Running 3% agarose gel after qRT-PCR (4) Analyze qRT-PCR data: Select those primers with one band in the gel picture and one peak in the dissociation curve for further experiments. At the same time, have a look at the Ct value to have a rough idea about how much RNA/cDNA is enough for the further
PCR em Tempo Real - Moodle USP: e-Disciplinas
edisciplinas.usp.br2. O corante SYBR Green I se liga a cada nova cópia de DNA dupla-fita. 3. Conforme a PCR progride, mais amplicons são criados. Como o SYBR Green I se liga a todo DNA dupla-fita, o resultado é um aumento na intensidade da fluorescência proporcional à quantidade de produto gerado pela PCR. Vantagens do SYBR Green I
StepOne and StepOnePlus Real-Time PCR Systems
tools.thermofisher.comreal-time PCR results • Long-life, LED-based optical system records fluorescence from FAM™/SYBR ® Green I, VIC ™/JOE™, and ROX™ dyes for gene expression analysis, pathogen quantitation, SNP genotyping, and presence/absence assays. The StepOnePlus system also accommodates the TAMRA™ dye • Both systems perform Fast PCR reactions in ...
PROTOCOL PowerUp SYBR Green Master Mix Guidelines for ...
tools.thermofisher.comMix using this simple protocol. In our own experiments, we found that PowerUp SYBR Green Master Mix outperformed other master mixes when comparing specificity, PCR efficiency, dynamic range, and precision over multiple targets. This protocol is for the comparison of PowerUp SYBR Green Master Mix to one other master mix using 20 µL reactions in
SsoAdvanced Universal SYBR Green Supermix
www.bio-rad.comSsoAdvanced universal SYBR ® Green supermix is a 2x concentrated, ready-to-use reaction master mix optimized for dye-based real-time PCR on any real-time PCR instrument (ROX-independent and ROX-dependent). It contains antibody-mediated hot-start Sso7d fusion polymerase, dNTPs, MgCl2, SYBR® Green I dye, enhancers, stabilizers, and a blend of ...
Real-Time PCR Applications Guide - Bio-Rad Laboratories
www.bio-rad.comPCR. It includes guidelines for designing the best real-time PCR assay for your experiments and explains how real-time PCR data are used in various applications. In Sections 5–7, we present sample protocols and data that demonstrate the use of real-time PCR in specific applications, namely, gene expression analysis, allelic